Lucy is a utility that prepares raw DNA sequence fragments for sequence assembly, possibly using the TIGR Assembler. The cleanup process includes quality assessment, confidence reassurance, vector trimming and vector removal. The primary advantage of Lucy over other similar utilities is that it is a fully integrated, stand alone program.
where
PUC19 and PUC19splice are the complete vector sequence file and a partial splice site sequence file.
atie.seq is a seq file and atie.qul is the corresponding quilt.
atie.2nd is a 2nd sequence file
use following command to make a copy of samples at your local test directory
where
PUC19 and PUC19splice are the complete vector sequence file and a partial splice site sequence file.
atie.seq is a seq file and atie.qul is the corresponding quilt.
atie.2nd is a 2nd sequence file
use following command to make a copy of samples at your local test directory
lucy -v PUC19 PUC19splice atie.seq atie.qul atie.2nd -debug lucy.info
wherePUC19 and PUC19splice are the complete vector sequence file and a partial splice site sequence file.
atie.seq is a seq file and atie.qul is the corresponding quilt.
atie.2nd is a 2nd sequence file
use following command to make a copy of samples at your local test directory
cp /usr/local/doc/lucy/PUC19splice PUC19splice
cp /usr/local/doc/lucy/PUC19 PUC19
cp /usr/local/doc/lucy/atie.2nd atie.2nd
cp/usr/local/doc/lucy/atie.seq atie.seq
cp /usr/local/doc/lucy/atie.qul atie.qul